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The 2nd reaction cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis. As drawn in Fig - https://www.bark.com/en/us/company/olis-clarity/96z8l/. 9, the response might also involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As revealed in Fig. 9, this could be because the H4B radical acts as an electron sink in a later point in the reaction, so regarding allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.